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UCB collectionThree cryopreserved and seven fresh UCB samples were ready. The cryopreserved samples were processed after Rubinstein's protocol.36 the fresh samples were collected from normal fullterm delivery placentas: Blood was drained in sterile flasks including 10 ml of citrate phosphate dextrose adenine (CPDA) together with 500 IU of heparin (Clarisco strenght, Schwarz Pharma, Milano). After extraction, UCB samples were stored at room hot and cold temperature and processed within 24 h.
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