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The ex vivo activation protocol was first validated in adult PBSC and then applied to PBSC from children with advanced cancers.We studied the opportunity benefits of adding IL12 to IL2 in PBSC cultures and evaluated various culture times. the effects of cultures on the number and function of progenitor cells were also analysed.PBSC were extracted from 10 cancer patients, Five adults and five children whose clinical quality are summarised in Table 1. All patients received GCSF before PBSC debt collection.
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