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Membrane vesicle fractions were treated with PIPLC as described or left unattended (See supplies and Methods) And GPIanchored/transmembrane proteins were separated from nonGPIlinked protein by Triton X114 extraction as in (each). Lower portion of this panel shows levels of CAIV in the AQ and DGs of control and PIPLCtreated fractions contingent on Western blotting with antirabbit CAIV (YDQR). Of the fold increase and reduce of CAIV in AQ and DGs, Respectively, Following PIPLC digestion and removal with Triton X114.
The waterway is found along a hillside village in Muntinlupa City. The waterway is situated at the main road of Tunasan, Muntinlupa metropolitan, Philippines. The main goal of the city council of Muntinlupa in enforcing the ban on use of plastic bags and Styrofoam is to clear all the city's waterways of plastic discards.
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