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Top of pageDiscussionMyeloablative chemotherapy and ASCT is just used to improve outcomes of patients with relapsed or poor prognosis lymphoma. and yet, Given the frequent desire for multiple apheresis procedures in order to attain the minimally acceptable CD34+ cell dose for ASCT, Cost and annoyance of autograft collection and cryopreservation are of concern.10 Immediate autograft processing and cryopreservation has been the standard policy at many centers with the assumption that this maximizes autograft viability.11 back in Calgary, Because of the increasing use of ASCT, vices on the healthcare system and the study published by Lazarus et al.9 representing feasibility of nextday cryopreservation, We changed policy in september 2000 and began storing autografts overnight before cryopreservation. This change in policy allowed single but more prolonged, High volume leukapheresis and efficient time application by laboratory personnel,
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For 6 min. at the conclusion of this first centrifugation step, Supernatant waste was peeled off the cell washer by fixing the flow at 100 ml min1. the remainder of the cell suspension was automatically agitated for 80 s. sometimes its nagging and it often brings me to my knees. normally tucks pads relieve the pain for a small moment and a heating pad does the same. I have passed 5 small staples and had a Cscan and was told the tissue was eroding where the staples were placed on the colon and there's not another tumor in this area.
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