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Cell analysisCell aspect, Viability and apoptosis rates of the cells were decided by a hemocytometer and staining with annexin and propidium iodide. Viable cells were decided by Trypan blue exclusion in all samples. Apoptosis rates and surface molecules were analyzed in 15 specimens by flow cytometry as described previously12 applying this antibodies: Antihuman CD34FITC (BD Pharmingen, north park, ca, united states), CD133PE (Miltenyi Biotech, BergischGladbach, uk), CD45FITC (Pharmingen) and as a consequence CD184 (AntiCXCR4, Pharmingen).
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