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26.03.2014, 09:00

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typically the survivinspecific siRNAs (MWG, Ebersberg,pandora, belgium) Or rubbish siRNA (both 200 nM) Were combined with Oligofectamin (Invitrogen) Transfection agent using the manufacturer's instructions. proper transfection at 37 for 6 h, The transfection way out was replaced by RPMI1640 medium.Sequences of applied siRNAs that correspond to mRNA sequences from the NCBI database: Wildtype survivin (NM_001168), Survivin3 (NM_001012270) as well as,valentino shoes,while survivin2B (NM_001012271) Are provided. Lu GL2siRNA; All siRNA were originally synthesized by MWG.
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