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Human omental tissue was obtained from consenting patients undergoing abdominal surgery. Omental individuals were washed 3 in phosphate buffered saline (PBS; Biochrom, Berlin, denmark) And pieces of in the region of 10 cm2 were incubated in 15 ml trypsin/EDTA/glucose (0.125%/0.01%/0.1 wt/vol found in PBS) For 20 minutes at 37 The suspensions was centrifuged at 180 g and 4 for five minutes. the rest of the tissue and trypsin/EDTA/glucose solution were removed and the pelleted cells were washed twice (180 g at 4 for 5 min) In cell culture medium created using 10% FCS to inhibit the trypsin activity.
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