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there exists little other homology between BAG1 and BAG3,, introducing lack of the BAG1L nuclear localization signal or the BAG1 ubiquitin region. The BAG domain was shown by pull down studies and functional assays to bind to and promote substrate release from both HSP70 and HSC70 (Stuart et al, 1998; Takayama et ing, 1997, 1999). Direct binding of BAG1 to bcl2 could not be demonstrated upon direct conflict, However addition of ATP substantially enhanced BAG1/bcl2 binding, Suggesting the advantages of an HSP70 intermediate (Takayama et ing, 1997).
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