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Realtime PCR analyses of bone OPG and RANKLThe metaphyseal element of the tibia was cut and homogenized using a Mixer Mill MM400 (Retsch, uk) To separate the mRNA. Total RNA was isolated using TRIzol following the maker's guidelines (Invitrogen, Rockville, md, united states of america). The concentration and purity of the RNA were driven by measuring the absorbance at 260 nm and 280 nm.
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