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NC from whole blood were mentioned, practicality assessed by Trypan blue exclusion, Diluted to 1 106 in Iscove's adjusted Dulbecco's medium (Gibco) And plated in backup at 1 105 in Methocult GF H4434 (Stem Cell solutions, vancouver, north america). system were incubated at 37 in humidified 5% CO2. Granulocyte colonies (CFUGM) Containing upwards of 50 cells were scored by inverted microscope on day 10.
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