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a suitable: the effect of Tlymphocyte preactivation with sCD3 mAb, PHA, or simply iCD3/iCD28 mAbS on gene transduction efficiencies. T lymphocytes were transduced with the scFvG250 chRec at days 2 after activation by 10 ng/mL sCD3 mAb, 1 PHA, or alternatively 1 immobilized CD3/CD28 mAb. The G250TAA cytolytic motion of the transduced lymphocytes on G250TAAPOS (charcoal box) combined with G250TAANEG (Black triangular) RCC target cells was measured in a 3hour 51Cr release assay at day 18 after lymphocyte activation.
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